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GluePCA Following transformation, cultures were grown to saturation in SC-Ura and subjected to a second selection in 200/1,000 ml (ZF and Ikaros-mimicry library/DMS library, as described above) SC-Ura/Ade/Met at OD 600 = 0.4 until reaching an OD 600 of 1.2–1.4
S-adenosylmethionine
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