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Briefly, yeast cells from the second round of sorting and displaying parental scFv R023, were stained with 10 nM biotinylated soto-p 7 /A*11 for 30 min, followed by the removal of unbound antigen and subsequent incubation with 100 nM non-biotinylated competitor (non-biotinylated soto-p 7 /A*03) for 2 h at room temperature
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