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It is a critical part of your injectable protocol, and we treat it with exactly that level of care
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An equal volume of 2 × urea sample buffer (150 mM Tris pH 6.8, 6 M Urea, 6% SDS, 40% glycerol, 100 mM DTT, 0.1% bromophenol blue) was added to the lysates and heated at 65°C for 5 minutes
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