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Step 2: Reconstitute Using Sterile Technique to Prevent Peptide Degradation Once you've calculated bacteriostatic water volume using your mix 5-Amino-1MQ calculator method, sterile reconstitution prevents the two failure modes that ruin peptides: microbial contamination and air injection

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After deparaffinization and antigen retrieval (5 min treatment at room temperature with 95% formic acid, followed by 30 min incubation in citrate buffer, pH 6.0, at 90 degrees C