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The Role of Glutathione and Its Precursors in Type 2 Diabetes

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EMSA assay was performed to examine the binding activity of TF-GT-1 and the mutated TF-GT-1 proteins with the site-directed mutagenesis of Cys 324 and Cys 347 into Ser residues in single residue mutagenesis (GT-1C324S or GT-1C347S), treated with GSNO (10 μM) or plus cPTIO (200 μM)