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Studies suggest that various molecular pathways are involved in IC/BPS, including the JUN N-terminal kinase (JNK) pathway, transient receptor potential (TRP) channels, activated mast cells, mucosal signalling, circadian rhythm regulation, inflammation and neural dysregulation

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In diet-induced obese (DIO) mouse models, 5-Amino-1MQ treatment produced significant reductions in body weight, white adipose tissue mass, and adipocyte cell size — without significant effects on food intake — suggesting a metabolic rather than appetite-suppressing mechanism