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Quantitative RT-PCR was performed on LightCycler 480 (Roche) using the LightCycler 480 High Resolution Melting Master Mix (Roche) and the following primers: cbiL-forward: 5’-GCGCGAGGCAGACGTGATCC-3’, cbiL -reverse: 5’-GACACCGGACCTCTCCCGCA-3’, cysG+cbiX-forward: 5’-TGTATTCCGCCCCGCTGTTGC-3’, cysG+cbiX-reverse: 5’-GAGCACTGCCGACGTGTCCC-3’, btuR-forward: 5’-GGAAGATGCTCTTCGGGCGCT-3’, btuR -reverse: 5’ -GCCTCAGGGTTCTCCGCAGC-3’, 16S-forward: 5’-GGGGCTTAACCCTGAGCGTGC-3’, 16S-reverse: 5’-TTCGCTCCCCACGCTTTCGC-3’

There are two files