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Our work focuses on dependence of the action of the DNA damaging anticancer drug cisplatin on metabolic regulation through p53/p21 axes and cellular thiamine status in human lung adenocarcinoma cells A549

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Brains were rapidly extracted and immersed in chilled cutting solution (250 mM sucrose, 26 mM NaHCO 3 , 10 mM D(+)-glucose, 4 mM MgCl 2 , 3 mM myo-inositol, 2.5 mM KCl, 2 mM sodium pyruvate, 1.25 mM NaH 2 PO 4 , 0.5 mM ascorbic acid, 0.1 mM CaCl 2 , and 1 mM kynurenic acid, pH 7.4, continuously oxygenated with 95% O 2 and 5% CO 2 )