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Colonies were used to inoculate 0.25 ml SC-ULH medium (1.47 g l −1 Synthetic Complete (Kaiser) Drop Out: Leu, His, Ura, 6.7 g l −1 yeast nitrogen base (without amino acids), 20 g l −1 d-(+)-glucose), pH set to 5.8 with hydrochloric acid) or SC-U medium (SC-ULH, 380 mg l −1 leucine, 76 mg l −1 histidine), respectively, in 96-deep-well plates equipped with a CR1296 sandwich cover (EnzyScreen)