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Wash with PBS one more time and add 500 μl of lysis buffer (0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5) on ice

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[] Since 1985, the FDA has authorised NAC as a 72-h oral regimen for treating acetaminophen overdose in the United States