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Blocks containing a single hemisphere from each animal were coronally sectioned at a thickness of 40 μm on a Leica cryostat, and stored in a cryoprotectant solution of primarily glycerol at − 20 °C until used

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To directly test this idea, we first applied bleomycin to animals and 5 days later administered intrapleural AAV-Cystatin A, tracked the animal’s weight and investigated lungs at day 21 post bleomycin instillment (Fig