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The mechanism of induction of phase II enzyme expression by plant polyphenols has been elucidated by Zoete and colleagues [233]

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The PGMRC1 (a.a.44–195 for in vitro studies and a.a.72–195 for crystallographic analyses and SV-AUC) cDNA fragment was amplified with PCR, digested with Bam HI and Sal I and then ligated into pGEX6P-1 (GE Healthcare)