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Poly(ADP-ribose) polymerase-1-dependent cardiac myocyte cell death during heart failure is mediated by NAD+ depletion and reduced Sir2alpha deacetylase activity

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After 96 h culture, cells were harvested and the viability was determined by a MTT (methylthiazolyldiphenyl-tetrazolium bromide) colorimetric procedure 55 and apoptosis by a flow cytometric method 56