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While in vitro equilibrations between redox couples such as roGFP2 and GSH/GSSG require minutes or even hours to reach a thermodynamic endpoint (ΔG = 0), enzyme-catalyzed thiol-disulfide exchange reactions usually occur very rapidly and either maintain or reach a steady state (ΔG ≠ 0) 7,31,32,60,63,67,78,79
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