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RT-qPCR analyses showed that none of the ATG genes were activated in the 2-h period following the transfer in the medium with no GSH, whereas strong activation occurred when all sulfur was depleted (Supplementary Fig
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The samples destined for GC/MS analysis were re-dried under vacuum desiccation for a minimum of 24 hours prior to being derivatized under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA)
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