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In some histological and electrophysiological experiments, AgRP-IRES-Cre or LepR-IRES-Cre mice were also crossed with the Cre-inducible tdTomato-reporter mouse (B6;129S6-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J, The Jackson Laboratory), allowing the visualization of AgRP or LepR neurons via expression of the tdTomato fluorescent protein

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Let’s discuss the two main vitamins mentioned – Biotin and B12