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therapure

Variant::90Gx3

Briefly, 50 µL of concentrated conditioned cell culture medium was added to 50 µL of LOX working solution containing LOX substrate, horseradish peroxidase, and Amplite horseradish peroxidase substrate and incubated at 37 °C for 1 h, during which fluorescence intensity was measured at Ex/Em = 540/590 nm by a fluorescence spectrophotometer