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Bosentan Added to Sildenafil Therapy in Patients with Pulmonary Arterial Hypertension

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The samples were then centrifuged at 18 000 g at 4 °C for 15 min, and 20 μl of the resulting supernatants was injected into the HPLC column for metabolite quantification, while the pellet was used for protein analysis using the BCA protein assay