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Apoptosis was then analysis by annexin V and 7-AAD staining

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Peaks detected in CE-TOFMS analysis were extracted using automatic integration software (MasterHands ver.2.17.1.11 developed at Keio University) 76 and those in CE-QqQMS analysis were extracted using automatic integration software (MassHunter Quantitative Analysis B.06.00 Agilent Technologies) in order to obtain peak information including m / z , migration time (MT), and peak area