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The assays were performed immediately at 37°C in a 96-well plate, and fluorescence readings were acquired with a SpectraMax® M2 Multimode Microplate Readers (Molecular Device, Sunnyvale, CA), using excitation of 535 nm and emission of 590 nm for a total of 15 kinetic cycles with an interval of 20 s per cycle
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[DOI] [PubMed] [Google Scholar] 123.Scolapio J.S., Camilleri M., Fleming C.R., Oenning L., Burton D., Sebo T.J., Batts K., Kelly D