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After additional washing in PBST, nanobodies were diluted four times ten gradients starting at 400 μg/mL concentration and were added to the wells and incubated for 2.5 h at 37 °C

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ML:30 ml

For example, the peptide PN159 (KLAL/MAP) is reported to bind claudin-4, destabilizing tight junctions and thereby increasing paracellular permeability (Bocsik et al., 2019)