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The next day, cells were collected in ice cold PBS, pelleted and sonicated in NP-40 lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40) with protease inhibitors (Roche)

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When researchers administered Epitalon to old rhesus monkeys (aged 20 to 27 years), nighttime melatonin levels increased significantly