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For the reanalysis, MBR (match between run) and smart profiling were enabled to create a spectral library from the DIA data

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In vitro KAT-1 can use many α-keto acids as amino group acceptors and although it has detectable activity on oxaloacetate and pyruvate, the specific activity on these two α-keto acids is very slow, making unlikely that they are physiological substrates for human KAT I [186]