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Type:Glutathione
Crystallization of Pt GSTF8 in presence of (±)-catechin and GSH Purified Pt GSTF8 in 30 mM Tris, pH 8, 200 mM NaCl, 1 mM EDTA was concentrated to 12.4 mg ml −1 using an Amicon Ultra-4 device (10 kDa cut-off) (Merck Millipore) and supplemented with 1 mM GSH and 25 mM (±)-catechin immediately before crystallization