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It contains a carboxyl group (which is in the deprotonated −COO − form under biological pH conditions), an amino group (which is in the protonated −NH + 3 form under biological pH conditions) located in α-position with respect to the carboxyl group, and an S -methyl thioether side chain, classifying it as a nonpolar, aliphatic amino acid

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These analyses were extended to analysis of covariance to adjust for the baseline HAM-D score

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