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Dose escalation studies were carried out in groups of 4 mice with matched doses of 0.1 µg, 1 µg, and 10 µg delivered at weeks 0 and 8 and bleeds were collected every 2 weeks for 18 weeks

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The presence of bands in the lanes corresponding to the DSIP-CBBBP, CBBBP, and DSIP constructs suggests successful expression of these fusion proteins