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To assess the impact of FOXO4-DRI on endothelial cell senescence, the senescent endothelial cells were treated with FOXO4-DRI, followed by immunofluorescence and Western blotting experiments

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In mice subjected to TBI, T1 weighted images taken with gadolinium contrast showed areas of hyper-intensity which indicate BBB disruption (Figures 1A and 1B, see asterisk in the TBI image which marks an area of BBB disruption)