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However, the existence of IGFBPs significantly reduces the levels of immunoreactive IGF-1 in samples, requiring multiple pre-treatment steps that reduce reproducibility and complicates interpretation of IGF-1 assay results

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Appetite Effects The question about appetite is interesting because the proposed mechanism could reasonably go either direction: Reduced lipogenesis and improved metabolic flexibility could produce secondary effects on appetite regulation NAD+ restoration has complex effects on hypothalamic appetite signaling Preclinical data do not consistently describe dramatic appetite changes In human use — were it to become clinically available — patient-reported appetite effects would be a relevant monitoring parameter, but the data do not currently support a strong a priori expectation