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Systemic L-buthionine -S-R-sulfoximine treatment increases plasma NGF and upregulates L-cys/L-cys2 transporter and gamma-glutamylcysteine ligase mRNAs through the NGF/TrkA/akt/Nrf2 pathway in the striatum

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The solutions were mixed and incubated at 25°C for about 5 min