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Incubation time should be adjusted by sample type: 60 minutes at 37°C for rat serum/plasma (high GSH-PX activity) and 90 minutes for rat brain or aged rat tissues (low activity)—prolonged incubation enhances signal without increasing non-specific binding

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[DOI] [PubMed] [Google Scholar] 52.Won CH, Park GH, Wu X, Tran TN, Park K, Park BS, et al