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To explore this hypothesis, we tested mitragynine ( 1 ), 7-hydroxymitragynine ( 2 ) and speciofoline ( 3 ) for µ-opioid receptor inhibition of forskolin-stimulated cyclic-AMP (cAMP) accumulation and for their ability to recruit β-arrestin-2 in CHO-K1 cells stably expressing the µ-opioid receptor
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However, the presence of conserved amino acids in the shared motif between Zot and zonulin lead these researchers to synthesize an octapeptide, now known as LA, with the same amino acid sequence as the NH 2 -terminus of human fetal zonulin, positions 8–15 (7)
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