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Quantification of different metabolites was performed with a liquid chromatography/tandem mass spectrometry (LC-MS/MS) method using a C18 column (maintained at 50 °C, Biocrates) for amino acids, ZORBAX Stable Bond CN (2.1 × 150 mm, 5 μm

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Although glutamate levels were normal in CSF and plasma, this does not exclude the possibility of deviant levels in the brain and a local effect