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Briefly, redox fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate (DCF-DA) (Sigma-Aldrich D6883) was used to estimate the ROS level in intact RBC

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The next day, the cells were spun down at 2 × g (Beckman Avant J-26 XP rotor JLA8.1000) for 30 min, washed once with 25 mL cold PBS and stored at −80 °C